Permafrost, or permafrost, consists of soil, rock, and sedimentary materials frozen in regions below 0°C for at least two consecutive years. Permafrost areas are fascinating with their microbial load. The sub-zero atmospheres of these areas allow microorganisms to maintain their viability for years or even centuries. Climate change indicates a serious risk of melting in the Arctic and other permafrost regions. Due to their microbial load-induced metabolic activities, melting permafrost can increase the release of greenhouse gases and disrupt the environmental balance. Microbial load is essential not only for the release of carbon gases but also for the increase of microbial-borne diseases. It is known that apparent microbial and vector-borne diseases have increased in the Arctic region.The melting of permafrost areas due to atmospheric warming, which in turn exposes new soils and microorganisms, has the potential to trigger significant changes in the biosphere.