Release Date: 2024-06-07

Natural and Powerful Antioxidant Source Propolis According to in Vitro Analysis Results

Ilhami Gulcin (Author), Ebubekir Izol (Author)

Release Date: 2024-06-07

Antioxidants are leading the way in the prevention of more than 100 diseases. They are divided into synthetic and natural antioxidants. Due to the harmful effects of synthetic antioxidants, studies on natural antioxidants have intensified. Propolis, which has the highest antioxidant properties among bee products, is a source of natural antioxidants. Propolis shows much stronger [...]

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    Work TypeBook Chapter
    Published inNatural Antioxidants and in Vitro Antioxidant Assays
    First Page1
    Last Page15
    DOIhttps://doi.org/10.69860/nobel.9786053359333.1
    Page Count15
    Copyright HolderNobel Tıp Kitabevleri
    Licensehttps://nobelpub.com/publish-with-us/copyright-and-licensing
    Antioxidants are leading the way in the prevention of more than 100 diseases. They are divided into synthetic and natural antioxidants. Due to the harmful effects of synthetic antioxidants, studies on natural antioxidants have intensified. Propolis, which has the highest antioxidant properties among bee products, is a source of natural antioxidants. Propolis shows much stronger antioxidant properties than many medicinal aromatic plants and other bee products. Therefore, in this chapter, the antioxidant potential of propolis, which is a natural and powerful antioxidant, is presented with current in vitro studies.

    Ilhami Gulcin (Author)
    PhD, Prof. Dr., Ataturk University
    https://orcid.org/0000-0001-5993-1668
    3Prof. Dr. İlhami Gülçin is one of the leading scientists in the world with his scientific studies in the field of antioxidants. In his studies, he has brought the antioxidant potential of many natural products to the world of science. He has hundreds of international articles in this field. He is still continuing many projects on natural antioxidants. He has also trained many scientists in the field of antioxidants.

    Ebubekir Izol (Author)
    PhD, Dr., Bingöl University
    https://orcid.org/0000-0003-0788-4999
    3Dr. Ebubekir İZOL is an important scientist who is an expert in the field of natural antioxidants. He researches the antioxidant properties of natural products and has published international articles in this field. He is also an international book editor and chapter author in this field. He is involved in many projects related to the determination of the biological activities of medicinal aromatic plants and other natural products. He also has many studies on the antioxidant potential and other biological activities of bee products.

    • İzol, E. (2023). “A Miraculous Bee Product:Propolis”, In Functional Medicine-Part 5, ed. Haspolat Y.K., Atabay A., Aşır F., Orient Publications, 15-24.

    • İzol, E. (2023). Bazı Arı Ürünlerinin (Bal, Polen, Propolis, Arı Sütü ve Arı Ekmeği) LC-MS/MS ile Sekonder Metabolitlerinin ve Biyolojik Aktivitelerinin Belirlenmesi. Doktora tezi. Atatürk Üniversitesi Fen Bilimleri Enstitüsü, Erzurum.

    • İzol, E. (2021). “Yenilikçi Arı Ürünleri ve Biyolojik Önemleri.” In Tarım Uygulamalarında Yenilikçi Yaklaşımlar, ed. İnci H., Kökten K., Iksad Publications, 77–116.

    • Yapıcı, İ., İzol, E., Tarhan, A. (2023). “Significant Bioactive Components in Bee Products”, In Bee and Bee Products, ed. İzol E., Koçyiğit M., Haspolat Y.K., Orient Publications, 1-15.

    • Yapıcı, İ., İzol, E. (2023). “Phytochemicals and their Bioavailability”, In Medicinal, Aromatıc Plants and Phytochemicals, ed. İzol E., Yılmaz M. A., Haspolat Y. K., Orient Publications, 43-60.

    • İzol, E. (2023). “The Place of Bee Products in Functional Medicine”, In Functional Medicine Part 2, ed. Haspolat Y.K., Atlı A., Aşır F., Orient Publications, 11-16.

    • İzol, E. (2021). “Yenilikçi Arı Ürünleri ve Biyolojik Önemleri.” In Tarım Uygulamalarında Yenilikçi Yaklaşımlar, ed. İnci H., Kökten K., Iksad Publications, 77–116.

    • İzol, E. (2022). “Arı Ürünlerinin Sağlıklı Beslenmedeki Önemi”, In Sağlıklı Yaşam ve Beslenme, ed. Haspolat Y.K., Ertuğrul S., Orient Publications, 313-323.

    • İzol, E. & Bengü, A.Ş. (2022). “Apiterapi ve Klinik Uygulamalar”, In Sağlık Bilimlerinde İnovatif Yöntemler, Teoriler ve Uygulamalar, ed. Bengü A.Ş., İzol E., Iksad International Publishing House,1, 21.

    • Izol, E. 2016. Determination of Heavy Metals and Seconder Metabolites of some Allium (Wild Garlic) Species by ICP-MS and LC-MS/ MS Investigation of Their Biological Activities. Dicle Üniversitesi, Fen Bil. Ens., Kimya ABD, Yüksek Lisans Tezi.

    • Bursal, E., Yılmaz, M. A., Izol, E., Türkan, F., Atalar, M. N., Murahari, M., ... &Ahmad, M. (2021). Enzyme inhibitory function and phytochemical profile of Inula discoidea using in vitro and in silico methods. Biophysical Chemistry, 277, 106629.

    • Karagecili, H., Izol, E., Kirecci, E., Gulcin, I. (2023). Determination of Antioxidant, Anti-Alzheimer, Antidiabetic, Antiglaucoma and Antimicrobial Effects of Zivzik Pomegranate (Punicagranatum) —A Chemical Profiling by LC-MS/MS). Life, 13, 735.

    • Inci, H., Izol, E., Yilmaz, M. A., Ilkaya, M., Bingöl, Z., & Gülçin, I. (2023). Comprehensive Phytochemical Content by LC/MS/MS and Anticholinergic, Antiglaucoma, Antiepilepsy, and Antioxidant Activity of Apilarnil (Drone Larvae). Chemistry & Biodiversity, 20(10), e202300654.

    • Karageçili, H., İzol, E., Kireçci, E. & Gülçin, İ. (2023). Antioxidant, antidiabetic, antiglaucoma, and anticholinergic effects of Tayfi grape (Vitis vinifera): A phytochemical screening by LC-MS/MS analysis. Open Chemistry, 21(1), 20230120.

    • Yılmaz, M.A., Cakir, O., İzol, E., Tarhan, A., Behçet, L., Zengin, G. (2023). Detailed Phytochemical Evaluation of a Locally Endemic Species (Campanula baskilensis) by LC-MS/MS and its In-Depth Antioxidant and Enzyme Inhibitory Activities. Chemistry&Biodiversity, 20(12), e202301182.

    • Izol, E., Temel, H., Yilmaz, M. A., Yener, I., Olmez, O. T., Kaplaner, E., ... &Ertas, A. (2021). A detailed chemical and biological investigation of twelve Allium species from Eastern Anatolia withchemometric studies. Chemistry&Biodiversity, 18(1), e2000560.

    • Gulcin, İ. (2020). Antioxidants and antioxidant methods: An updated overview. Archives of toxicology, 94(3), 651-715.

    • Gülcin, I. (2012). Antioxidant activity of food constituents: an overview. Archives of toxicology, 86, 345-391.

    • Gülçin, İ. (2010). Antioxidant properties of resveratrol: A structure–activity insight. Innovative food science & emerging technologies, 11(1), 210-218.

    • Tinggi, U. (2008). Selenium: its role as antioxidant in human health. Environmental health and preventive medicine, 13, 102-108.

    • Gülçin, İ., Bursal, E., Şehitoğlu, MH, Bilsel, M. ve Gören, AC (2010). Erzurum, Türkiye'den propolisin liyofilize sulu ekstraktının polifenol içerikleri ve antioksidan aktivitesi. Gıda ve Kimyasal Toksikoloji , 48 (8-9), 2227-2238.

    • Güven, L., Ertürk, A., Yılmaz, M. A., Alwasel, S., & Gülçin, İ. (2024). Alchemilla pseudocartalinica Juz: Phytochemical Screening by UPLC-MS/MS, Molecular Docking, Anti-oxidant, Anti-diabetic, Anti-glaucoma, and Anti-Alzheimer Effects. Records of Natural Products.

    • Baxter, H., Puri, B., Harborne, J. B., Hall, A., & Moss, G. P. (1998). Phytochemical dictionary: a handbook of bioactive compounds from plants. CRC press.

    • Markulin, L., Corbin, C., Renouard, S., Drouet, S., Gutierrez, L., Mateljak, I., ... & Lainé, E. (2019). Pinoresinol–lariciresinol reductases, key to the lignan synthesis in plants. Planta, 249, 1695-1714.

    • Blois, M.S. (1958). Antioxidant determinations by the use of a stable free radical. Nature, 181(4617), 1199-1200.

    • Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(9-10), 1231-1237.

    • Benzie, I. F. F. and Strain, J. J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: the FRAP assay. Analytical Biochemistry, 239, 70-76.

    • Prior, R.L., and Cao, G. (2000). Antioxidant phytochemicals in fruits and vegetables: diet and health implications. HortScience, 35(4), 588-592.

    • Singleton, V. L., & Rossi Jr, J. A. (1965). Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents. American Journal of Enology and Viticulture, 16(3), 144-158.

    • Fogliano, V., Verde, V., Randazzo, G., & Ritieni, A. (1999). Method for measuring antioxidant activity and its application to monitoring the antioxidant capacity of wines. Journal of agricultural and food chemistry, 47(3), 1035-1040.

    • Liu, R., Xu, Y., Chang, M., Tang, L., Lu, M., Liu, R., ... & Wang, X. (2021). Antioxidant interaction of α-tocopherol, γ-oryzanol and phytosterol in rice bran oil. Food Chemistry, 343, 128431.

    • Apak, R., Özyürek, M., Güçlü, K., & Çapanoğlu, E. (2016). Antioxidant activity/capacity measurement. 1. Classification, physicochemical principles, mechanisms, and electron transfer (ET)-based assays. Journal of agricultural and food chemistry, 64(5), 997-1027.

    • Valkonen, J., & Kuusi, T. (1997). Spectrophotometric and Fluorometric Measurements of Hydroperoxides by Oxidation of Fe2+ in the Presence of Xylenol Orange. Free Radical Biology and Medicine, 22(5), 883-889.

    • Winston, G. W., Regoli, F., & Dugas, A. J. (1998). Foy's Principles of Medicinal Chemistry, 5th ed., Williams and Wilkins, Baltimore, MD, 485.

    • Ghiselli, A., Serafini, M., Natella, F., & Scaccini, C. (1995). Total Antioxidant Capacity as a Tool to Assess Redox Status: Critical View and Experimental Data. Free Radical Biology and Medicine, 18(1), 97-107.

    • Singleton, V. L., Orthofer, R., & Lamuela-Raventós, R. M. (1999). Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin-Ciocalteu Reagent. Methods in Enzymology, 299, 152-178.

    • Kohen, R., Nyska, A., & Kohen, C. (1999). Antioxidant Characteristics of Propolis. Journal of Toxicology and Environmental Health, Part A, 66(7), 689-703.

    • Whitehead, T. P., Thorpe, G. H., & Maxwell, S. R. (1992). Enhanced Chemiluminescent Assay for Antioxidant Capacity in Biological Fluids. Analytica Chimica Acta, 266(2), 265-277.

    • Dinis, T. C. P., Madeira, V. M. C., & Almeida, L. M. (1994). Action of Phenolic Derivatives (Acetaminophen, Salicylate, and 5-Aminosalicylate) as Inhibitors of Membrane Lipid Peroxidation and as Peroxyl Radical Scavengers. Archives of Biochemistry and Biophysics, 315(1), 161-169.

    • Tubaro, A., Dri, P., Delbello, G., Zilli, C., Della Loggia, R., & Dri, P. (1998). The Croton Oil Ear Test Revisited. Agents and Actions, 45(1-2), 5-12.

    • Miller, N. J., & Rice-Evans, C. A. (1971). Factors Influencing the Antioxidant Activity Determined by the ABTS•+ Radical Cation Assay. Free Radical Research, 25(5), 315-323.

    • Wayner, D. D., Burton, G. W., & Ingold, K. U. (1985). The Relative Contributions of Vitamin E, Urate, Ascorbate, and Proteins to the Total Peroxyl Radical-Trapping Antioxidant Activity of Human Blood Plasma. Biochimica et Biophysica Acta (BBA) - General Subjects, 924(3), 408-419.

    • Necip, A., Demirtas, I., Tayhan, S. E., Işık, M., Bilgin, S., Turan, İ. F., ... & Beydemir, Ş. (2024). Isolation of phenolic compounds from eco‐friendly white bee propolis: Antioxidant, wound‐healing, and anti‐Alzheimer effects. Food Science & Nutrition, 12(3), 1928-1939.

    • Karagecili, H., Yılmaz, MA, Ertürk, A., Kızıltaş, H., Güven, L., Alwasel, SH, & Gülçin, İ. (2023). LC-MS/MS kullanılarak Berdav propolisinin kapsamlı metabolit profili: Antioksidan, antikolinerjik, antiglokom ve antidiyabetik etkilerin belirlenmesi. Moleküller , 28 (4), 1739.

    • Nazari‐Bonab, H., Jamilian, P., Radkhah, N., Zarezadeh, M. ve Ebrahimi‐Mameghani, M. (2023). Propolis takviyesinin antioksidan durumunu iyileştirmedeki etkisi: Kontrollü klinik çalışmaların sistematik bir incelemesi ve meta-analizi. Fitoterapi Araştırması , 37 (9), 3712-3723.

    • Nayak, G., Sahu, A., Bhuyan, SK, Bhuyan, R., Kar, D. ve Kuanar, A. (2023). Doğu Hindistan'ın farklı tarımsal bölgelerinden propolis etanolik ekstraktının ve yağının antioksidan aktivitesi üzerine karşılaştırmalı bir çalışma. Biyokataliz ve Tarımsal Biyoteknoloji , 50, 102685.

    • Sá, SHG, Mazzocato, MC, Saliba, ASM, Alencar, SM ve Favaro-Trindade, CS (2023). Püskürtmeli kurutma, püskürtmeli soğutma ve her iki tekniğin kombinasyonu kullanılarak kapsüllenmiş Brezilya kırmızısı propolis ekstraktının salınımının, stabilitesinin ve antioksidan aktivitesinin değerlendirilmesi. Gıda Araştırması Uluslararası , 164 , 112423.

    • Paula, VB, Estevinho, LM, Cardoso, SM ve Dias, LG (2023). Propolisin Antioksidan Kapasitesini Değerlendirmeye Yönelik Karşılaştırmalı Yöntemler: Farklılıkları Açıklamaya Yönelik Bir Girişim. Moleküller , 28 (12), 4847.

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