Cystic Echinococcosis and Molecular Diagnosis

Yunus Emre Beyhan (Author)

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Cystic echinococcosis (CE) is a parasitic disease caused by the larval form of Echinococcus granulosus. It predominantly affects the liver but can also impact the lungs, kidneys, spleen, brain, bones, and heart. CE is prevalent in regions with low socio-economic status and is associated with significant health and economic burdens due to medical costs and [...]

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    Work TypeBook Chapter
    Published inMolecular Approaches in Medicine
    First Page1
    Last Page20
    DOIhttps://doi.org/10.69860/nobel.9786053359524.1
    Page Count20
    Copyright HolderNobel Tıp Kitabevleri
    Licensehttps://nobelpub.com/publish-with-us/copyright-and-licensing

    Cystic echinococcosis (CE) is a parasitic disease caused by the larval form of Echinococcus granulosus. It predominantly affects the liver but can also impact the lungs, kidneys, spleen, brain, bones, and heart. CE is prevalent in regions with low socio-economic status and is associated with significant health and economic burdens due to medical costs and reduced livestock productivity. Diagnosis typically involves radiological and serological methods, and treatment primarily consists of surgery, though drug therapy and less invasive procedures like PAIR are also used. Prevention focuses on controlling animal slaughter practices, improving public hygiene, and providing education on the disease. The molecular approach in cystic echinococcosis (CE) involves identifying and differentiating species within the Echinococcus genus using DNA-based methods. PCR and its variations, such as PCR-RFLP, RAPD-PCR, LAMP, mPCR, and DNA sequencing, are employed to determine genetic diversity and specific genotypes. These methods are highly sensitive and specific, aiding in accurate diagnosis and epidemiological studies. The E. granulosus complex comprises several genotypes (G1-G10), with G1 being the most significant for human infections. Molecular techniques, including sequencing of mitochondrial genes (cox1, nad1) and nuclear genomic regions (ITS1), provide insights into the parasite’s genetic diversity, host specificity, and epidemiology. Studies in various regions have identified multiple genotypes, highlighting the importance of accurate molecular typing for effective epidemiological studies and control measures. In summary, CE is a significant parasitic disease with widespread implications. Effective diagnosis, treatment, and prevention require a multifaceted approach, incorporating clinical, radiological, and molecular techniques.

    Yunus Emre Beyhan (Author)
    Professor, Van Yuzuncu Yil University
    https://orcid.org/0000-0002-1696-4803
    3Prof. Dr. Yunus Emre Beyhan received his PhD in molecular parasitology from the University of Ondokuz Mayis (Samsun, Turkey) and then began working at the National Parasitology Reference Laboratories, Public Health Science Institute of Turkey, as a researcher. During that time, his main interests included serological and molecular parasitology and zoonotic parasitic diseases. In 2014, he was appointed as an Assistant Professor at the Department of Medical Parasitology, Yüzüncü Yıl University Faculty of Medicine, Van, Turkey. Dr. Beyhan is a member of the Parasitology Society of Turkey and the Turkish Microbiology Society. In 2022, he received the title of Professor in the same field. He has attended three different courses in parasitology at the Centers for Disease Control and Prevention in the USA. He was a visiting researcher at the Department of Parasitology at La Sapienza University in Italy and the Department of Parasitology at the Medical University of Gdansk in Poland. He is also a coordinator of the Ministry of Health Zoonosis National Committee of Cystic Echinococcosis Board. Dr. Beyhan has given presentations in various international cities, including those in the Czech Republic, Sofia, Krakow, Amsterdam, Kuala Lumpur, Freiburg, and Lisbon. He is the author of 68 publications and has given 65 presentations.

    • Altintas, N. (2003). Past to present: echinococcosis in Turkey. Acta Trop, 85(2): 105-112

    • Alvarez Rojas, C. A., Romig, T., Lightowlers. M. W. (2014). Echinococcus granulosus sensu lato genotypes infecting humans review of current knowledge. Int J Parasitol, 44(1): 9-18.

    • Bart, J.M., Morariu, S., Knapp, J., et al. (2006). Genetic typing of Echinococcus granulosus in Romania. Parasitol Res, 98(2): 130-137.

    • Beyhan, Y. E., Çobanoğlu, U., Çelik, S., et al. (2020). Molecular characterization of human lung and liver cystic echinococcosis isolates in Van Province, Turkey. Acta tropica, 206, 105451

    • Boubaker, G., Macchiaroli, N., Prada, L., et al. (2013). A multiplex PCR for the simultaneous detection and genotyping of the Echinococcus granulosus complex. PLoS Neglec Trop Dis, 7(1), e2017.

    • Bowles, J., McManus, D.P. (1993). Rapid discrimination of Echinoccus specises and strains using a polymerase chain reaction-based RFLP method. Mol Biochem Parasitol, 57: 231–240.

    • Chen, F., Liu, L., He, Q., et al. (2019). A multiplex PCR for the identification of Echinococcus multilocularis, E. granulosus sensu stricto and E. canadensis that infect human. Parasitol, 146(12), 1595-1601.

    • Çetinkaya, Z., Çiftçi, I.H., Demirel, R., et al. (2005). A sero-epidemiologic study on cystic echinococcosis in Midwestern region of Turkey. Saudi Med J, 26(2): 350-351.

    • Dybicz, M., Gierczak, A., Dąbrowska, J., et al. (2013). Molecular diagnosis of cystic echinococcosis in humans from central Poland. Parasitol Int, 62(4): 364-367.

    • Eckert J., Schantz P.M., Gasser R.B. (2001). Geographic distribution and prevalence. In: Eckert J, Gemmell MA, Meslin FX, Pawlowski ZS (Editors). WHO/ OIE Manual on Echinococcosis in Humans and Animals: a Public Health Problem of Global Concern, Chapter 4: 101-143

    • Ergin, S., Saribas, S., Yuksel, P., et al. (2010). Genotypic characterisation of Echinococcus granulosus isolated from human in Turkey. Afr J Microbiol Res, 4(7), 551-555.

    • Eryıldız, C., Sakru, N. (2012). Molecular characterization of human and animal isolates of Echinococcus granulosus in the Thrace Region, Turkey. Balkan Med J, 29: 261–267.

    • Hemphill, A., Stadelmann, B., Rufener, R., et al. (2014). Treatment of echinococcosis: albendazole and mebendazole–what else?. Parasite, 21.

    • Kim, H.J., Yong T.S., Shin M.H., et al. (2020). Phylogenetic characteristics of Echinococcus granulosus sensu lato in Uzbekistan. Korean J Parasitol, 58: 205–210.

    • Kinkar L., Laurimäe T., Acosta-Jamett G., et al. (2018). Distinguishing Echinococcus granulosus sensu stricto genotypes G1 and G3 with confidence: a practical guide. Infect Genet Evol, 64: 178–184

    • Lymbery A.J. (2017). Phylogenetic pattern, evolutionary processes and species delimitation in the genus Echinococcus. Adv Parasitol, 95: 111–145.

    • Loos-Frank, B., Grencis, R. K. (2016). Parasitic worms. In: The Biology of Parasites (Lucius, R., Loos-Frank, B., Lane, R. P., et al. (Ed.), 182. Springer-verlag, Berlin.

    • Macin S., Orsten S., Samadzade R., et al. (2021). Human and animal cystic echinococcosis in Konya, Turkey: molecular identification and the first report of E. equinus from human host in Turkey. Parasitol Res, 120: 563–568.

    • Malik, A. A., Bari, S. U., Malik, A. A., et al. (2019). Biology of the Echinococcus. Human Abdominal Hydatidosis, 1-13.

    • Mandal S., Mandal M.D. (2012). Human cystic echinococcosis: epidemiologic, zoonotic, clinical, diagnostic and therapeutic aspects. Asian Pac J Trop Med, 5(4): 253-260

    • Marinova I., Spiliotis M., Wang J., et al. (2017). Molecular characterization of Echinococcus granulosus isolates from Bulgarian human cystic echinococcosis patients. Parasitol Res, 116(3): 1043-1054.

    • McManus D.P., Gray D.J., Zhang W., et al. (2012). Diagnosis, treatment, and management of echinococcosis. BMJ. 344: e3866.

    • McManus D.P., Thompson R.C. (2003). Molecular epidemiology of cystic echinococcosis. Parasitol, 127: 37-51.

    • Mogoye B. K., Menezes C.N., Wong M. L., et al. (2013). First insights into species and genotypes of Echinococcus in South Africa. Vet Parasitol, 23; 196(3-4): 427-432.

    • Mohaghegh M. A., Yousofi-Darani H., Jafarian A. H., et al. (2019). Isolated Human and Livestock Echinococcus granulosus Genotypes Using Real-Time PCR of cox1 Gene in Northeast Iran. Acta Parasitol, 64(3): 679-685.

    • Moro P.L., Schantz P.M. (2006). Cystic echinococcosis in the Americas. Parasitol Int, 55: 181-186.

    • Nabarro L.E., Amin Z., Chiodini P. L. (2015). Current Management of Cystic Echinococcosis: A Survey of Specialist Practice. Clin Infect Dis, 60(5): 721-728.

    • Sadjjadi S. M. (2006). Present stituation of echinococcosis in the Middle East and Arabic North Africa. Parasitol Int, 55: 197-202

    • Seimenis A. (2003). Overview of the epidemiological situation on echinococcosis in the Mediterranean region. Acta Trop, 85: 191-195.

    • Šoba B., Gašperšič Š., Keše D., Kotar T. (2020). Molecular Characterization of Echinococcus granulosus sensu lato from Humans in Slovenia. Pathogens, 9(7): 562.

    • Tamarozzi, F., Hou, A., Morales, M. L., et al. (2017). Prevalence and risk factors for human cystic echinococcosis in the Cusco region of the Peruvian highlands diagnosed using focused abdominal ultrasound. Am J Trop Med Hyg, 96(6): 1472.

    • Thompson R. C. A. (2017). Biology and systematics of Echinococcus. Adv Parasitol, 95: 65–109.

    • Thompson R. C. A. (2008). The Taxanomy, Phylogeny and Transmission of Echinococcus. Exp parasitol, 119: 439-446.

    • Torgerson, P. R., Budke, C. M. (2003). Echinococcosis–an international public health challenge. Res Vet Sci, 74(3), 191-202.

    • Urach Monteiro D., de Azevedo M. I., Weiblen C, et al. (2016). Echinococcus granulosus sensu stricto, Echinococcus canadensis (G7), and Echinococcus ortleppi in fertile hydatid cysts isolated from cattle in Southern Brazil. Acta Trop., 164: 41-44.

    • Velasco-Tirado, V., Alonso-Sardón, M., Lopez-Bernus, A., et al. (2018). Medical treatment of cystic echinococcosis: systematic review and meta-analysis. BMC Infect Dis, 18, 1-19.

    • Vuitton D. A., McManus D. P., Rogan M. T., et al. (2020). World Association of Echinococcosis. International consensus on terminology to be used in the field of echinococcoses. Parasite, 27: 41

    • WHO Informal Working Group on Echinococcosis. (2003). International classification of ultrasound images in cystic echinococcosis for application in clinical and field epidemiological settings. Acta Trop, 85: 253-261.

    • WHO/OIE Manual on Echinococcosis. (2001). Echinococcosis in Humans and Animals: A Public Health Problem of Global Concern. World Organisation for Animal Health (Office Inter- national des Epizooties) and World Health Organisation.

    • Wassermann M., Mackenstedt U., Romig T. (2014). A loop-mediated isothermal amplification (LAMP) method for the identification of species within the Echinococcus granulosus complex. Vet Parasitol, 200: 97-103.

    • Woolsey, I. D., Miller, A. L. (2021). Echinococcus granulosus sensu lato and Echinococcus multilocularis: A review. Res Vet Sci, 135, 517-522.

    • Yazar S. Kayseri’de Kistik Ekinokokkozisin Son Altı Yıldaki Durumu. Türkiye Parazitol Derg 2005; 29: 241-243.

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