American Foulbrood (AFB) is one of the most important infections affecting all life stages of honey bee (Apis mellifera L.) brood. It is a infection that can be seen at any time of the year when honey bee brood is present. The causative agent of the disease is Paenibacillus larvae (P.larvae), a spore-forming. AFB is the most devastating bacterial infection of honey bees (Apis mellifera L.) (1). Infection is not only dangerous and lethal for honey bee larvae. Loss of offspring due to increased egg-laying activity in the colony can no longer be replaced by the queen bee. As a result, the population decreases, which can lead to the collapse of the whole colony. The number of adult bees in the colony constantly decreases and the colony strength decreases.